Capability of Autologous Platelet Rich Plasma to Alleviate Experimentally Induced Diabetic Nephropathy in Adult Male Albino Rat (Light and Electron Microscopic Study)

Background: Diabetic nephropathy is a common complication of diabetes. In prior studies platelet-rich plasma (prp) ameliorated diabetic cardiac injury and diabetic neuropathy.
Aim: This work aimed to evaluate the capability of platelet-rich plasma to alleviate experimentally induced diabetic nephropathy in adult male albino rat.
Material and methods: Forty adult male albino rats were used in this study. Rats were distributed into equal four groups.
Group I (control): rats were redistributed into two equal subgroups:
- Subgroup a: rats received no treatment for six weeks.
- Subgroup b: each rat was intraperitonially injected by a single dose of citrate buffer (1ml) then kept without any treatment for six weeks.
Group II (diabetes): each rat received 0.5 mL/kg prp, twice weekly by subcutaneous injection for six weeks.
Group III (prp): each rat received a single intraperitoneal injection of 55mg/kg Streptozotocin (Sz), then after confirmation of diabetes they kept without any treatment for six weeks.
Group IV (diabetes+ prp): each rat received a single intraperitoneal injection of Sz as in group III and from the day of confirmation of diabetes, prp was injected as in group II.

Results: The diabetic group showed that the cells of the renal tubules were having cytoplasmic vacuoles with scanty apical microvilli and scanty basal mitochondria. Meanwhile, the renal corpuscles showed hypertrophied glomeruli and enlarged mesangial cells. Diabetic rats that received prp showed nearly intact renal tubules and renal corpuscles.

Conclusion: Autologous platelet rich plasma could alleviate the histological changes of diabetic nephropathy in adult male albino rats.