VITAMIN D3 ADMINISTRATION BEFORE AND AFTER DETORSION COULD SALVAGE THE TESTICULAR ENDOCRINE FUNCTION IN AN EXPERIMENTAL MODEL OF TESTICULAR TORSION/DETORTION

Background: Testicular torsion is one of the emergencies affecting mostly adolescent males, resulting in sub-fertility if not
rapidly and efficiently managed. Oxidative stress, inflammatory response as well as immunological reactions were implicated in its pathogenesis. Recently interest in studying the non-skeletal effects of vitamin D is growing, many researches indicates its antioxidant, antiinflammatory and immuno-modulatory effects. Aim of the Work: Investigating the possible conservative effect of vitamin D3 treatment on testicular endocrinal function in testicular torsion/detorsion rat model, and elucidating its possible underlying mechanism. Materials and Methods: 24 young adult male albino rats,
weighing 120-160 g., were randomly allocated into 3 groups, 8 in each group, Sham operated (SHAM), Testicular torsion/detorsion (T/D) and Testicular torsion/detorsion; vitamin D3 treated (T/D; D3) groups. All rats were subjected to measurement of absolute and relative testicular weight (ATW and RTW, respectively), assessment of testicular endocrinal function by serum total testosterone and inhibin B levels. In addition to determination of serum antisperm antibody
(AsAb). Testicular tissue was examined for oxidative stress markers; malondialdeyde (T. MDA) and glutathione peroxidase (T, GPx), as well as inflammatory marker; myeloperoxidase (T. MPO). Results: Testicular T/D resulted in significant reduction in ATW, RTW, serum testosterone and inhibin B levels with significant elevation in serum AsAb, T. MDA and T. MPO when compared with SHAM group. On treatment with vitamin D3, RTW was significantly increased as compared with T/D group but still significantly less than SHAM group. Serum testosterone level was significantly increased when compared with both SHAM and T/D groups. Inhibin B was significantly increased as compared with T/D group but still not
normalized as being significantly less than SHAM group. Serum AsAb, T. MDA and T. MPO were significantly decreased when compared with T/D group, being normalized for T. MDA but still significantly higher for serum AsAb and T. MPO when compared with SHAM group, however, T. GPx was significantly increased as compared with both SHAM and T/D groups.
Conclusion: Vitamin D3 could retrieve the testicular endocrinal dysfunction induced by Torsion/Detorsion by its antioxidant, antiinflammatory and immuno-modulatory effects.