NEW PRODUCTION METHOD OF BACTERIAL L-ASPRAGINASE ENZYME FROM DIFFERENT SOIL ENVIRONMENTS IN EGYPT AS ANTICANCER AGENT

Background: Acute lymphoblastic leukemia (ALL) is a hematologic cancer that preponderantly falls out in children betwixt 2 and 10 years of age. L-asparaginase is a constitutional element of management for long-suffering with ALL. L-asparagine degrading enzymes are necessary for the treatment of auxotrophic cancers such as acute lymphoblastic leukaemia. Auxotrophic cancers have not the ability to synthesize asparagine but normal cells can synthesize it. Asparagine degrading enzymes include asparaginase enzyme. The aim of the study: our study was concerned with isolation and screening of bacterial producing L-asparaginase enzyme as anticancer agent for treatment of auxotrophic cancers from different soil environments. Also, concerned with determination of environmental and physiological factors affecting the growth of some L-asparaginase producing bacterial isolates. Characterization of L-asparaginase enzyme was also included in our study.
The type of the study: Screening experimental study. Methodology: In our study some bacterial isolates were analyzed for production of L-asparaginase on mineral asparagine agar selective media (MAA). These bacterial isolates which showed positive growth on MAA utilized the asparagine as the sole metabolic source of carbon and nitrogen for their growth. Direct Nesslerization test was used for detection and screening of the presence of bacterial asparagine degrading enzymes via their ability to produce ammonia from degrading the asparagine in different dilutions of soil samples. Results and discussion: The optimal environmental and physiological factors affecting growth of these positive isolates were PH 7.3 at temperature 37c in aerobic conditions. The morphological and the biochemical tests revealed that Bacillus subtilis and Bacillus anthracis were the major positive bacterial producing L-asparaginase isolates of soil samples collected from different soil environments. Conclusion: Characterization of bacterial L-asparaginase production as anticancer agent in Egypt included activators of the enzyme production :0.5 g/l KCL,0.5 g/l MGSO4,1.0 g/l FeSO4,0.1 g/l ZnSO4 at PH 7.3 at temperature 37 under aerobic conditions.