Detection of adhesion genes in Enterobacter spp. Isolated from clinical cases in Al-najaf city

Document Type : Original Article

Authors

1 Department of Medical Laboratory Techniques, Technical Institute, Al-Furat Al-Awsat Technical University, Kufa, Iraq.

2 Islamic university Najaf.

3 Islamic university Najaf

4 Al-Furat Al-Awsat Hospital.

Abstract

Background and Aim of the study: Enterobacter spp. is also an important nosocomial pathogen that causes bacteremia and infections of the lower respiratory tract, urinary tract, and burns, as well as endocarditis, septic arthritis, and skin and soft tissue infections. A sample )204) was isolated from different cases and these isolates distributed by 98 isolated from urine, 42 isolated from wound , 35 isolated from diabetic foot, 11 isolated from vaginal swab and 18 isolated from burns in Al-Najaf city(Al-Sadr Medical City, AL-Forat Hospital) and identification of bacterial isolates was performed using biochemical testing and Vitek 2 system, PCR amplification technique was used to investigation of predominance, showed that (23) isolates were Enterobacter spp. After incubation periods, the results showed that (77.07%) samples showed bacterial growth and (22.92%) showed no growth. Enterobacter spp. identified in (29.84%) the bacterial growth adhesion genes and fimH and the aggregation genes esgA and csgD among Enterobacter spp.
Results: The results of PCR breeding technique showed that (26%) and (17%) bacterial isolates carried fimH and fimA genes, while (13%) bacterial isolates carried esgA and csgD genes.

Keywords

Main Subjects

Files

Share

How to cite

Statistics