The new production method of bacterial L-asparaginase enzyme from different soil environments in Egypt as an anticancer agent

Background
Asparagine degrading enzymes are necessary for the treatment of auxotrophic cancers such as acute lymphoblastic leukemia. Auxotrophic cancers do have not the ability to synthesize asparagine but normal cells can synthesize it. Asparagine degrading enzymes include the asparaginase enzyme.
The aim of the study
our study was concerned with the isolation and screening of bacterial-producing L-asparaginase enzyme as an anticancer agent for the treatment of auxotrophic cancers from different soil environments. Also, concerned with the determination of environmental and physiological factors affecting the growth of some L-asparaginase-producing bacterial isolates. Characterization of the L-asparaginase enzyme was also included in our study.
The type of the study
Screening experimental study.
Methodology
In our study, some bacterial isolates were analyzed for the production of L-asparaginase on mineral asparagine agar selective media(MAA). These bacterial isolates which showed positive growth on MAA utilized asparagine as the sole metabolic source of carbon and nitrogen for their growth. Direct Nesslerization test was used for detection and screening of the presence of bacterial asparagine degrading enzymes via their ability to produce ammonia from degrading the asparagine in different dilutions of soil samples.
Conclusion
Characterization of bacterial L-asparaginase production as an anticancer agent in Egypt included activators of the enzyme production:0.5 g/l KCL,0.5 g/l MGSO4,1.0 g/l FeSO4,0.1 g/l ZnSO4 at PH 7.3 at temperature 37 under aerobic conditions.