Erythropoietin, Myoinositol and Metformin modulate Insulin Sensitivity Indices, Pancreatic Beta Cell Mass and hepatocellular changes in a Rat Model of PCO

Articles in Press

Document Type : Original Article

Authors

1 department of histology, faculty of medicine, sohag university

2 department of physiology, faculty of medicine, sohag university.

3 department of physiology , faculty of medicine, sohag university

4 department of physiology, faculty of medicine, sohag university

Abstract

Abstract:
Background: Polycystic ovary (PCO) is a common health problem in females throughout their reproductive age. Common symptoms of PCO are hyperandrogenemia, infertility, and menstrual disorders. Metabolic disorders like insulin resistance and steatotic liver are also common.
Objective: This study aimed to compare the potential therapeutic role of erythropoietin, myoinositol, and the widely used metformin drug against hyperinsulinemia and hepatic injury of letrozole-induced PCOS model in rats.
Materials and methods: fifty female Wistar rats were classified equally into five groups: The control group, PCO model group: PCO model was induced by letrozole in a dose of 0.5 mg/kg daily for 3 weeks. Then rats with PCO were administrated for the following 21 days: erythropoietin (EPO/PCO) group, myoinositol (MYO/PCO) group, or metformin (MET/PCO) group. Biochemical and histological studies on the liver and pancreas were done. Immunohistochemical staining with GLUT-1 and insulin was done.
Results: PCOS rats developed insulin resistance with pancreatic β cell degeneration, hepatocellular injury and upregulation of hepatic GLUT-1.
Conclusion: erythropoietin was most effective in restoring B cell mass, decreasing hyperinsulinemia associated with letrozole -induced PCOS model, and attenuating hepatocellular degeneration via reduction of stress induced hepatic GLUT-1.

Keywords

Ain Shams Medical Journal

Articles in Press, Accepted Manuscript
Available Online from 01 October 2024

Files

Share

How to cite

Statistics