Confirmation between Microscopy and Pcr Technique for Differentiation between Entamoeba Histolytica and Entamoeba Dispar among Children in Duhok City, Iraq

Document Type : Original Article

Author

Department of Medical Laboratory Sciences, College of Health Sciences, Duhok University, Duhok, Iraq

Abstract

Aims: The current study aimed to confirm between microscopy and PCR techniques to differentiate between Entamoeba
histolytica and Entamoeba dispar and to determine the prevalence of amoebiasis among children in Duhok City, Iraq.
Materials and Methods: We conducted this study from June to August 2021. A total of 300 fresh stool samples were collected
from children with a history of diarrhea/dysentery from both sexes.
Results: Depending on the microscopic examination of stool samples, the prevalence rate of amoebiasis among children in
the Duhok Province was high (34.0%). A higher prevalence rate was recorded according to gender in girls than in boys (18.0%
& 16.0%) respectively, and among the age groups reported a high prevalence rate in the age groups between (4-6 years & 7-9
years) (15.67% & 12.33%), respectively. The lowest infection rate was recorded in the age groups (1-3 years and 10-12 years)
(4.0% and 2.0%). This variation was statistically not significant at p < 0.05. Finally, the PCR results show that similar results
were recorded positive for Entamoeba histolytica (92.0%). By microscopy, just a few samples were positive with Entamoeba
histolytica, and by PCR, they were negative for Entamoeba histolytica and positive for Entamoeba dispar (8.0%), and one
sample was found positive with Entamoeba histolytica and Entamoeba dispar.
Conclusion: To lower the prevalence rate of infection among the community by supplying clean water, a suitable sanitation
system, and supporting efforts to educate people about health.

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